Lindane is an organochlorinated insecticide that was used to treat crops and livestock during the 1940s-70s. Due to its overuse, it has now become a persistent environmental pollutant. In 2009, lindane was added to the Stockholm Convention on Persistent Organic Pollutants (POPs), an international treaty that aims to reduce the use of POPs. Although lindane has since been banned from the agricultural industry in several countries, major waste stockpiles still exist and it remains an ingredient for second line treatment of lice and scabies. Lindane is a suspected carcinogen and endocrine disruptor, but the cellular mechanisms that are involved in these effects are still largely unknown. Using a Saccharomyces cerevisiae (yeast) deletion library, we have explored these mechanisms. Pools of deletion strains were exposed for 5 and 15 generations to a concentration of lindane (160uM) known to inhibit the growth of wild-type yeast by 20% (IC20). The growth of the different strains was monitored and through PCR amplification, the unique sequences of each deletion strain were prepared for microarray analysis, which helped identify mutants sensitive, unaffected, and resistant to the toxicity of lindane. Computational analyses such as FunSpec will be used to narrow down the mutants to pursue for further testing. A fluorescence-based flow cytometry method will be used to confirm the sensitivity or resistance of the mutants chosen. This experiment will provide more information on the toxicity of lindane, and ultimately give vital insight on human toxicity, due to the conservation of cellular processes between yeast and humans.