The Purification And Characterization Of 1C6 That Recognizes Canine CD28

CD28 is a molecule on the surface of T cells, involved in the T-cell costimulatory pathway and functions in allograft rejection and tolerance. 1C6 is a hyrbidoma antibody that was produced in cell culture. It was purified via affinity chromatography. The material was quantitated using A280 spectrophotometry. The characterization of this newly purified 1C6 was compared to existing lots of 1C6 via ELISA, SDS-PAGE, flow cytometry, and a mixed leukocyte reaction. We expect expect the existing and newly purified lots to be similar in the tests. In the mixed leukocyte reaction, we expect 1C6 to block CD28.